Bücher für Medizin, Wissenschaft und Studium

Zell- und Molekularbiologie

This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable as an introduction for students. Topics covered range from the basics—such as measuring the concentrations of macromolecules—through considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.

This companion website provides a set of computer simulations that can be used to visualize important concepts presented in the book, offering a better understanding of binding and rate curves and fitting data - http://kinetics.cshl.edu

1. Performing Quantitative Experiments with Biomolecules
2. Affinity Constants
3. Cooperativity in Binding
4. Rate Constants for Binding and Dissociation
5. Kinetics of Regulation and Cooperativity in Binding Reactions
6. Constants for a Two-step Binding Reaction
7. Data Analysis
Appendix 1: Equation Derivations
Appendix 2: Resources and References
Index

James A. Goodrich, University of Colorado, Boulder; Jennifer F. Kugel, University of Colorado, Boulder