Bücher für Medizin, Wissenschaft und Studium

Bioinformatik
Molekularbiologie

Next-generation DNA sequencing (NGS) technology has revolutionized biomedical research, making complete genome sequencing an affordable and frequently used tool for a wide variety of research applications. Bioinformatics methods to support DNA sequencing have become a critical bottleneck for many researchers and organizations wishing to make use of NGS technology. This book provides a thorough introduction to the necessary informatics methods and tools for operating NGS instruments and analyzing NGS data. The book also provides extensive reference to best-practice bioinformatic methods for the most commonly used NGS technologies and applications. The book also includes reference to, and guidance, on the setup and use of essential software for NGS data analysis. This is the first book of its kind to address the informatics needs of scientists who wish to take advantage of the explosion of research opportunities offered by new DNA sequencing technologies.

Contents

About the Authors
Preface
Acknowledgments
1. Introduction to DNA Sequencing
Stuart Brown
2. History of Sequencing Informatics
3. Visualization of Next-Generation Sequencing Data
Phillip Ross Smith, Kranti Konganti, and Stuart Brown
4. DNA Sequence Alignment
Efstratios Efstathiadis
5. Genome Assembly Using Generalized de Bruijn Digraphs
D. Frank Hsu
6. De Novo Assembly of Bacterial Genomes from Short Sequence Reads
Silvia Argimón and Stuart Brown
7. Genome Annotation
Steven Shen
8. Using NGS to Detect Sequence Variants
Jinhua Wang, Zuojian Tang, and Stuart Brown
9. ChIP-seq
Zuojian Tang, Christina Schweikert, D. Frank Hsu, and Stuart Brown
10. RNA Sequencing with NGS
Stuart Brown, Jeremy Goecks, and James Taylor
11. Metagenomics
Alexander Alekseyenko and Stuart Brown
12 High-Performance Computing in DNA Sequencing Informatics
Efstratios Efstathiadis and Eric Peskin
Glossary
Index

Genetik - Genome - Molekularbiologie - Bioinformatik